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Assessment of genetic diversity in Aloe germplasm accessions from India using RAPD and morphological markers

Authors:

NMC Nayanakantha ,

Plant Science Department, Rubber Research Institute of Sri Lanka, Dartonfield, Agalawatta, LK
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BR Singh,

G.B. Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttaranchal, IN
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AK Gupta

G.B. Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttaranchal, IN
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Abstract

Eleven Aloe germplasm accessions; A. vera, A. perryi, A. lotus, A. zeylanicum and seven strains of A. vera available at the Defense Agricultural Research Laboratory (DARL), Pithoragarh, and Medicinal and Aromatic Plant Research Development Centre (MRDC), Pantnagar, Uttaranchal, India were subjected to Random Amplified Polymorphic DNA (RAPD) analysis in relation to morphometric parameters for estimating the extent of diversity within and between species. Morphological evaluation of the 11 accessions for selected characters showed qualitative variation among the accessions studied. The RAPD analysis revealed comparable inter and intra species variation. A total of 192 bands were amplified with 7 primers. Out of 192 bands amplified, 89% was polymorphic and 10.9% was unique to a particular accession which made it distinct from all other accessions. Maximum similarity of 61% was observed between DARL 1 and DARL 3 (A. vera) and minimum similarity of 6.8 % was observed between A. lotus and A. perryi. Thus, Aloe accessions maintained at DARL showed high genetic diversity. The RAPD profiles would be useful in genetic improvement and authentication of species and genotype of this medicinally and economically important genus.

Key words: Aloe vera; cluster analysis; genetic similarity

DOI: 10.4038/cjsbs.v39i1.2315

Cey. J. Sci. (Bio. Sci.) 39 (1): 1-9, 2010

DOI: http://doi.org/10.4038/cjsbs.v39i1.2315
How to Cite: Nayanakantha, N., Singh, B. & Gupta, A., (2010). Assessment of genetic diversity in Aloe germplasm accessions from India using RAPD and morphological markers. Ceylon Journal of Science (Biological Sciences). 39(1), pp.1–9. DOI: http://doi.org/10.4038/cjsbs.v39i1.2315
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Published on 12 Oct 2010.
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