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Reading: A simple and rapid DNA extraction method for Cyanobacteria and Monocots

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A simple and rapid DNA extraction method for Cyanobacteria and Monocots

Authors:

Dhammika Magana-Arachchi ,

Institute of Fundamental Studies, Hantana Road, Kandy, LK
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Rasika Wanigatunge

Institute of Fundamental Studies, Hantana Road, Kandy, LK
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Abstract

The isolation of DNA from biological samples is a crucial step in the process of DNA-based molecular biological assays. In this study, we present a simple method to extract and purify genomic DNA from different sample types (standard cyanobacteria, cyanobacteria in water samples and leaves of bamboo plants) which would be suitable for any molecular analyses. In this method, cyanobacteria are lysed by three sequential freezing and heating followed by enzymatic treatment with lysozyme and proteinase K. The extraction and purification of DNA was achieved with the lysing and nuclease inactivating properties of the chaotropic agent guanidium isothiocyanate and the nucleic acid binding properties of silica particles. The DNA extraction method yielded high quality reproducible DNA. The technique described here is a rapid, robust and cost effective method suitable for the extraction of DNA from any source for routine molecular biological assays.  

Key Words: Boom’s method, guanidine thiocyanate, silica, Polymerase Chain Reaction (PCR)

DOI: http://dx.doi.org/10.4038/cjsbs.v40i1.3407

CJSBS 2011; 40(1): 59-63

How to Cite: Magana-Arachchi, D. & Wanigatunge, R., (2011). A simple and rapid DNA extraction method for Cyanobacteria and Monocots. Ceylon Journal of Science (Biological Sciences). 40(1), pp.59–63. DOI: http://doi.org/10.4038/cjsbs.v40i1.3407
Published on 24 Aug 2011.
Peer Reviewed

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