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Research Articles

Development and characterization of tri- and tetra-nucleotide polymorphic microsatellite markers for skipjack tuna (Katsuwonus pelamis)

Authors:

Sudath T Dammannagoda ,

Biogeosciences Discipline, Faculty of Science and Engineering, Queensland University of Technology, GPO Box 2434, Brisbane, AU
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Vincent Chand,

Biogeosciences Discipline, Faculty of Science and Engineering, Queensland University of Technology, GPO Box 2434, Brisbane, AU
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Peter B Mather

Biogeosciences Discipline, Faculty of Science and Engineering, Queensland University of Technology, GPO Box 2434, Brisbane, AU
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Abstract

Skipjack tuna (SJT), Katsuwonus pelamis is the largest tuna fishery in all the major oceans around the world, and the largest marine fishery in Sri Lanka. Knowledge of genetic population structure and effective population size of SJT in the Indian Ocean and other major oceans, however, is still lacking for improved management practices and conservation strategies. We developed microsatellite genetic markers using SJT found around Sri Lanka in the Indian Ocean, and characterized one tri- and seven tetra-nucleotide microsatellite loci isolated from enriched genomic libraries from SJT, to provide tools for addressing both conservation and fisheries management strategies. Analysis of these eight microsatellite markers in two populations of SJT from eastern Sri Lanka (n = 44) and the Maldives Islands (n = 53) showed that all eight microsatellites were polymorphic with an average number of alleles per locus of 11.80 (range 5–27). Expected heterozygosities at marker loci ranged from 0.450 to 0.961. No significant linkage disequilibrium was detected among any loci pairs. These markers are currently being used to characterize population structure and extent of natural gene flow in SJT populations from the eastern and western Indian Ocean.

DOI: http://dx.doi.org/10.4038/cjsbs.v41i1.4533

Ceylon Journal of Science (Bio. Sci.) 41 (1): 11-17, 2012

How to Cite: Dammannagoda, S.T., Chand, V. & Mather, P.B., (2012). Development and characterization of tri- and tetra-nucleotide polymorphic microsatellite markers for skipjack tuna (Katsuwonus pelamis). Ceylon Journal of Science (Biological Sciences). 41(1), pp.11–17. DOI: http://doi.org/10.4038/cjsbs.v41i1.4533
Published on 01 Aug 2012.
Peer Reviewed

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