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Induction of L-form Bacteria from Bacillus thuringiensis

Authors:

PWHKP Daulagala ,

Department of Botany, Faculty of Natural Sciences, Kandy Regional Centre, The Open University of Sri Lanka, Polgolla,, LK
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EJ Allan

School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 3UU, Scotland,, GB
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Abstract

Induction of L-form bacteria from Bacillus thuringiensis was investigated with the long-term view of their use as targeted biological control agents within plants. L-forms were successfully induced from B. thuringiensis subspecies israelensis 36-3 by preparing pour plates of exponential cultures in L-phase medium (LPM) containing μg ml-1 cephalosporin C. The induced L-forms were then subcultured and maintained in L-phase medium supplemented with inactivated mycoplasma screened 5% (v/v) horse serum (5HS) with a combination of 225 μg ml-1 cephalosporin C and 0.6 mg ml-1 penicillin G, using the push block method. Induced L-forms showed typical features with pleiomorphic cells that contained granular particles and small vacuoles but no parasporal bodies. These L-forms were capable of growing on solidified media but failed to grow in liquid media.

Ceylon Journal of Science (Bio. Sci.) 41 (2): 137-144, 2012

DOI: http://dx.doi.org/10.4038/cjsbs.v41i2.5383

How to Cite: Daulagala, P. & Allan, E., (2013). Induction of L-form Bacteria from Bacillus thuringiensis. Ceylon Journal of Science (Biological Sciences). 41(2), pp.137–144. DOI: http://doi.org/10.4038/cjsbs.v41i2.5383
Published on 03 Apr 2013.
Peer Reviewed

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