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Immunoepidemiology of Plasmodium vivax Merozoite Surface Protein-4 (PVMSP-4) in Sri Lanka: A Preliminary Study

Authors:

Upadari Dissanayake,

Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka, LK
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Sajani Dias,

Department of Zoology, University of Colombo, Sri Lanka, LK
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Hannah Polson,

Laboratoire de Vaccinologie Parasitaire, Institut Pasteur, Paris, France, FR
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Shirley Longacre,

Laboratoire de Vaccinologie Parasitaire, Institut Pasteur, Paris, France, FR
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Preethi V Udagama-Randeniya

Department of Zoology, University of Colombo, Sri Lanka, LK
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Abstract

Natural antibody (IgG + IgM) responses to putative vaccine candidate, Plasmodium vivax Merozoite Surface Protein-4, in acute vivax malaria patients in endemic and non-endemic areas of Sri Lanka, were assayed by indirect ELISA using PvMSP-4 baculovirus derived recombinant protein. The study populations were from two vivax malaria endemic areas, Anuradhapura and Kataragama and from a non-endemic area, Colombo. PvMSP4 appeared to be immunogenic in all test populations with a prevalence of 80%, 62% and 62% from Colombo, Anuradhapura and Kataragama, respectively. A significantly higher prevalence and antibody magnitude was recorded for non-endemic Colombo, but with no significant differences between individuals previously not exposed to malaria and those with prior exposure. A positive trend in responders from Kataragama was evident between the antibody prevalence and previous exposure. We previously reported the antibody response to PvMSP-1 (p19 and p42) using the same battery of sera, and when comparisons were drawn, more than 80% from each test area responded to at least one of the three antigens tested. Importantly, 7%, 11% and 4% of individuals from Colombo, Anuradhapura, and Kataragama, respectively, preferentially recognized PvMSP-4. Responders to MSP-1p42 and p19 in paired combination showed higher prevalence compared with combinations of MSP-1 with MSP-4.

doi: 10.4038/cjsbs.v37i1.499

Cey. J. Sci. (Bio. Sci.) 37 (1): 97-105, 2008

Keywords: Plasmodium vivax 
How to Cite: Dissanayake, U. et al., (2009). Immunoepidemiology of Plasmodium vivax Merozoite Surface Protein-4 (PVMSP-4) in Sri Lanka: A Preliminary Study. Ceylon Journal of Science (Biological Sciences). 37(1), pp.97–105. DOI: http://doi.org/10.4038/cjsbs.v37i1.499
Published on 16 Jun 2009.
Peer Reviewed

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