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X-Ray induced changes in biochemical and histochemical parameters in the testis of male wild Indian house rate, Rattus rattus

Authors:

Kalyan Brata Santra ,

Department of Zoology, Daulatpur High School, Daulatpur, Dakshin Dinajpur, West Bengal, IN
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Chanchal Kumar Manna

Endocrinology Laboratory, Department of Zoology, University of Kalyani, West Bengal, IN
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Abstract

To observe the complementary effects, whole body of the male wild Indian house rat, Rattus rattus was irradiated with X-rays at 100 r, 200 r, 300 r and 500 r (single dose) doses. The changes in the histophysiological parameters of the testis were observed after 2, 7, 15 and 30 days of post treatment. Determination of biochemical parameters: ascorbic acid, cholesterol, acid and alkaline phosphatases in the testicular tissues indicated that concentrations of ascorbic acid and cholesterol (2 to 15 days post treatment) had increased while acid and alkaline phosphatases (2-30 days) had decreased. Changes in these parameters were observed in all four X-ray treated groups (100 r, 200 r, 300 r and 500 r). The histochemical study of the different X-ray treated groups clearly showed the intense accumulation of lipid substances and depletion of acid, alkaline phosphatases, 5-3-HSDH and 17-HSDH in the testicular tissues of 7 to 30 day - post treated groups exposed to 300 r and 500 r X-irradiation. In view of the changes observed in the testicular tissues, it is likely that a single dose of X- ray may cause certain histophysiological changes in the testis of wild rodents at least for certain periods. As a single dose of X-rays of 200r or 300r was sufficient to cause sterility in male rats, this may be included as a controlling agent that adversely effect the fertility of the rodent pests. Possible mode of action is discussed.

Key Words: rodent pest; acid and alkaline phosphatases; lipid; fertility; spermatogenesis

DOI: 10.4038/cjsbs.v38i2.1857

Cey. J. Sci. (Bio. Sci.) 38 (2): 39-49, 2009

How to Cite: Santra, K.B. & Manna, C.K., (2010). X-Ray induced changes in biochemical and histochemical parameters in the testis of male wild Indian house rate, Rattus rattus. Ceylon Journal of Science (Biological Sciences). 38(2), pp.39–49. DOI: http://doi.org/10.4038/cjsbs.v38i2.1857
Published on 26 Apr 2010.
Peer Reviewed

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