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Siderophore mediated metal uptake by Pseudomonas fluorescens and its comparison to Iron (III) chelation

Author:

Aditi Bhattacharya

HOD Microbiology, Dr. Rafiq Zakaria Campus, Maulana Azad College, Aurangabad, IN
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Abstract

Siderophores are iron chelating substances that are also known to bind metals such as Molybdenum and Lead. The pigment produced by P. fluorescens is a siderophore whose ability to bind to different metal ions was investigated in this study. Pigment production was increased in the presence of Sodium, Potassium, Lead, Molybdenum and Cadmium. Siderophores were detected using CAS assay and catechol - siderophores by Arnow assay. Peak changes and changes in extinction coefficient values are indicators of complex formation. Maximum difference was observed in siderophore free Iron and Iron-siderophore complexes. Some changes were also noted for siderophore- metal complexes for all metals included in this study. Thus siderophore of P. fluorescens shows a tendency to form complexes with almost all metals tested.

Key words: pigment; CAS assay; metal ions; complex formation; peak changes; molar extinction coefficient.

DOI: 10.4038/cjsbs.v39i2.3001

Cey. J. Sci. (Bio. Sci.) 39 (2): 147-155, 2010

How to Cite: Bhattacharya, A., (2011). Siderophore mediated metal uptake by Pseudomonas fluorescens and its comparison to Iron (III) chelation. Ceylon Journal of Science (Biological Sciences). 39(2), pp.147–155. DOI: http://doi.org/10.4038/cjsbs.v39i2.3001
Published on 21 Apr 2011.
Peer Reviewed

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