Induction of L-form bacteria from Bacillus thuringiensis was investigated with the long-term view of their use as targeted biological control agents within plants. L-forms were successfully induced from B. thuringiensis subspecies israelensis 36-3 by preparing pour plates of exponential cultures in L-phase medium (LPM) containing μg ml-1 cephalosporin C. The induced L-forms were then subcultured and maintained in L-phase medium supplemented with inactivated mycoplasma screened 5% (v/v) horse serum (5HS) with a combination of 225 μg ml-1 cephalosporin C and 0.6 mg ml-1 penicillin G, using the push block method. Induced L-forms showed typical features with pleiomorphic cells that contained granular particles and small vacuoles but no parasporal bodies. These L-forms were capable of growing on solidified media but failed to grow in liquid media.
Ceylon Journal of Science (Bio. Sci.) 41 (2): 137-144, 2012
How to Cite:
Daulagala, P. & Allan, E., (2013). Induction of L-form Bacteria from Bacillus thuringiensis . Ceylon Journal of Science (Biological Sciences) . 41 ( 2 ) , pp . 137–144 . DOI: http://doi.org/10.4038/cjsbs.v41i2.5383