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Reading: Methods for Analyzing Diversity of Microbial Communities in Natural Environments

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Methods for Analyzing Diversity of Microbial Communities in Natural Environments

Authors:

Md Fakruddin ,

Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, BD
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Khanjada Shahnewaj Bin Mannan

Center for Food and Waterborne Diseases, ICDDR B, Dhaka, BD
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Abstract

Difficulties in cultivating most of the microorganisms limit our ability to study microbial ecosystems. Molecular methods are valuable tools for investigating the diversity and structure of bacterial communities. These techniques can be used on culturable as well as non-culturable bacteria. Cultivation independent techniques based on nucleic acids extracted from the environment provide information on community structure and diversity. Analyses of DNA can determine the numbers of different genomes. Ribosomal RNA (rRNA) or rDNA (genes coding for rRNA) fingerprinting, probing and sequencing can be used to detect and identify organisms. The combination of different methods that complement each other is a useful strategy for monitoring changes of microbial communities in natural ecosystems.

DOI: http://dx.doi.org/10.4038/cjsbs.v42i1.5896

Ceylon Journal of Science (Bio. Sci.) 42 (1): 19-33, 2013

How to Cite: Fakruddin, M. & Mannan, K.S.B., (2013). Methods for Analyzing Diversity of Microbial Communities in Natural Environments. Ceylon Journal of Science (Biological Sciences). 42(1), pp.19–33. DOI: http://doi.org/10.4038/cjsbs.v42i1.5896
Published on 30 Jun 2013.
Peer Reviewed

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